Elisa Kit Safety Requirements

1. Avoid direct contact with stop solution and substrates A and B. In case of contact with these liquids, rinse with water as soon as possible.

2. Do not eat, drink, smoke or use cosmetics during the experiment.

3. Do not take any components of the kit with your mouth.

4. Reagents should be stored according to label instructions and brought to room temperature before use. The diluted standard should be discarded and cannot be stored.

5. The slats that are not used in the experiment should be immediately put back in the packaging bag and stored in an airtight container to avoid deterioration.

6. Other reagents not in use should be packaged or covered. Do not mix reagents from different batches. Use before warranty.

7. Use disposable tips to avoid cross-contamination. Avoid using pipettes with metal parts when aspirating Stop Solution and Substrate A and B.

8. Use a clean plastic container to prepare the cleaning solution. Mix the components and samples in the kit thoroughly before use.

9. When washing the ELISA plate, it should be fully patted dry, and do not put the absorbent paper directly into the ELISA reaction well to absorb water.

10. Substrate A should evaporate and avoid opening the lid for a long time. Substrate B is sensitive to light, avoid prolonged exposure to light. Avoid contact with hands, toxic. The OD value should be read immediately after the experiment is completed.

11. Reagents should be added in the same order to ensure that all reaction plate wells are incubated for the same time.

12. The incubation operation was carried out according to the time, amount and sequence of liquid addition indicated in the instructions.